GRLF1

ARHGAP35
Identifiers
Aliases	ARHGAP35, GRF-1, GRLF1, P190-A, P190A, p190ARhoGAP, p190RhoGAP, Rho GTPase activating protein 35
External IDs	OMIM: 605277; MGI: 1929494; GeneCards: ARHGAP35
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 2K85, 3C5H, 3FK2
Gene location (Human) Chr. Chromosome 19 (human) Band 19q13.32 Start 46,860,997 bp End 47,005,077 bp
Gene location (Mouse) Chr. Chromosome 7 (mouse) Band 7|7 A2 Start 16,227,644 bp End 16,348,918 bp
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in Brodmann area 23 endothelial cell middle temporal gyrus postcentral gyrus entorhinal cortex superior vestibular nucleus left testis pars compacta superior frontal gyrus right testis Top expressed in saccule otic placode genital tubercle otic vesicle tail of embryo dentate gyrus of hippocampal formation granule cell visual cortex primary visual cortex superior frontal gyrus secondary oocyte More reference expression data BioGPS More reference expression data
Gene ontology Molecular function DNA binding RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription corepressor activity GTP binding DNA-binding transcription repressor activity, RNA polymerase II-specific phospholipid binding GTPase activator activity GTPase activity nucleotide binding lipid binding Cellular component cytoplasm cytosol actin cytoskeleton nucleus cytoskeleton cell projection plasma membrane membrane ciliary basal body Biological process regulation of transcription, DNA-templated negative regulation of Rho protein signal transduction negative regulation of transcription by RNA polymerase II transcription, DNA-templated neural tube closure regulation of cell shape forebrain development camera-type eye development negative regulation of transcription, DNA-templated regulation of small GTPase mediated signal transduction signal transduction negative regulation of vascular permeability positive regulation of GTPase activity axon guidance axonal fasciculation regulation of actin polymerization or depolymerization positive regulation of neuron projection development cell migration central nervous system neuron axonogenesis mammary gland development establishment or maintenance of actin cytoskeleton polarity cellular response to extracellular stimulus regulation of actin cytoskeleton organization wound healing, spreading of cells positive regulation of cilium assembly regulation of axonogenesis neuron projection guidance Sources:Amigo / QuickGO
Orthologs Databases NCBI: entry; OMA: entry Species Human Mouse Entrez 2909 232906 Ensembl ENSG00000160007 ENSMUSG00000058230 UniProt Q9NRY4 Q91YM2 RefSeq (mRNA) NM_004491 NM_024342 NM_172739 RefSeq (protein) NP_004482 NP_766327 Location (UCSC) Chr 19: 46.86 – 47.01 Mb Chr 7: 16.23 – 16.35 Mb PubMed search
Wikidata
View/Edit Human View/Edit Mouse

Glucocorticoid receptor repression factor 1 (or GRF-1) is a protein that in humans is encoded by the ARHGAP35 gene (previously GRLF1). Preliminary evidence suggests that it functions to repress transcription of the glucocorticoid receptor, though this remains uncertain without further evidence.

Function

The human glucocorticoid receptor DNA binding factor, which associates with the promoter region of the glucocorticoid receptor gene (hGR gene), is a repressor of glucocorticoid receptor transcription. The amino acid sequence deduced from the cDNA sequences show the presence of three sequence motifs characteristic of a zinc finger and one motif suggestive of a leucine zipper in which 1 cysteine is found instead of all leucines. The GRLF1 enhances the homologous down-regulation of wild-type hGR gene expression. Biochemical analysis suggests that GRLF1 interaction is sequence specific and that transcriptional efficacy of GRLF1 is regulated through its interaction with specific sequence motif. The level of expression is regulated by glucocorticoids.

Further reading

• Nakajima D, Okazaki N, Yamakawa H, Kikuno R, Ohara O, Nagase T (Jun 2002). "Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones". DNA Research. 9 (3): 99–106. doi:10.1093/dnares/9.3.99. PMID 12168954.
• Warriar N, Pagé N, Govindan MV (Aug 1996). "Expression of human glucocorticoid receptor gene and interaction of nuclear proteins with the transcriptional control element". The Journal of Biological Chemistry. 271 (31): 18662–71. doi:10.1074/jbc.271.31.18662. PMID 8702520.
• Tikoo A, Czekay S, Viars C, White S, Heath JK, Arden K, Maruta H (Oct 2000). "p190-A, a human tumor suppressor gene, maps to the chromosomal region 19q13.3 that is reportedly deleted in some gliomas". Gene. 257 (1): 23–31. doi:10.1016/S0378-1119(00)00387-5. PMID 11054565.
• Nagase T, Kikuno R, Hattori A, Kondo Y, Okumura K, Ohara O (Dec 2000). "Prediction of the coding sequences of unidentified human genes. XIX. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro". DNA Research. 7 (6): 347–55. doi:10.1093/dnares/7.6.347. PMID 11214970.
• Beausoleil SA, Jedrychowski M, Schwartz D, Elias JE, Villén J, Li J, Cohn MA, Cantley LC, Gygi SP (Aug 2004). "Large-scale characterization of HeLa cell nuclear phosphoproteins". Proceedings of the National Academy of Sciences of the United States of America. 101 (33): 12130–5. Bibcode:2004PNAS..10112130B. doi:10.1073/pnas.0404720101. PMC 514446. PMID 15302935.
• Rush J, Moritz A, Lee KA, Guo A, Goss VL, Spek EJ, Zhang H, Zha XM, Polakiewicz RD, Comb MJ (Jan 2005). "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells". Nature Biotechnology. 23 (1): 94–101. doi:10.1038/nbt1046. PMID 15592455. S2CID 7200157.
• Barberis D, Casazza A, Sordella R, Corso S, Artigiani S, Settleman J, Comoglio PM, Tamagnone L (Oct 2005). "p190 Rho-GTPase activating protein associates with plexins and it is required for semaphorin signalling" (PDF). Journal of Cell Science. 118 (Pt 20): 4689–700. doi:10.1242/jcs.02590. PMID 16188938. S2CID 15559113.
• Holinstat M, Knezevic N, Broman M, Samarel AM, Malik AB, Mehta D (Jan 2006). "Suppression of RhoA activity by focal adhesion kinase-induced activation of p190RhoGAP: role in regulation of endothelial permeability". The Journal of Biological Chemistry. 281 (4): 2296–305. doi:10.1074/jbc.M511248200. PMID 16308318.
• Oh JH, Yang JO, Hahn Y, Kim MR, Byun SS, Jeon YJ, Kim JM, Song KS, Noh SM, Kim S, Yoo HS, Kim YS, Kim NS (Dec 2005). "Transcriptome analysis of human gastric cancer". Mammalian Genome. 16 (12): 942–54. doi:10.1007/s00335-005-0075-2. PMID 16341674. S2CID 69278.
• Olsen JV, Blagoev B, Gnad F, Macek B, Kumar C, Mortensen P, Mann M (Nov 2006). "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks". Cell. 127 (3): 635–48. doi:10.1016/j.cell.2006.09.026. PMID 17081983. S2CID 7827573.
• Mammoto T, Parikh SM, Mammoto A, Gallagher D, Chan B, Mostoslavsky G, Ingber DE, Sukhatme VP (Aug 2007). "Angiopoietin-1 requires p190 RhoGAP to protect against vascular leakage in vivo". The Journal of Biological Chemistry. 282 (33): 23910–8. doi:10.1074/jbc.M702169200. PMID 17562701.

External links

• GRLF1+protein,+human at the U.S. National Library of Medicine Medical Subject Headings (MeSH)
• GRLF1 Info with links in the Cell Migration Gateway Archived 2014-12-11 at the Wayback Machine

This article incorporates text from the United States National Library of Medicine, which is in the public domain.