Talk:Protein complex
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Ribosome in the lead
[edit]Ribosome is not a multiprotein complex, and not an enzyme. Ribosome is an RNA-protein complex (scaffold is mainly rRNA) and due to the catalytic site position, ribosome is a ribozyme. Should this article be moved to "biomolecular complex"? Because then one could include any stable complex determined by computational or structural means and may include DNA (nucleosome=DNA + protein), RNA (ribosome, spliceosome, telomerase,...= RNA + protein), protein, lipid (transmembrane protein complexes usually have some essential lipids bound), sugars (glycosylation of various proteins in complexes),... Kazkaskazkasako (talk) 10:23, 15 October 2013 (UTC)
List of papers about stoichiometry determination
[edit]An article Protein stoichiometry has been proposed for deletion. The text makes no sense, but I thought that the attached list of measurement methods may contain useful sources, so rescued it here as a backup.
- Absolute Quantification (AQUA)[1]
- Multiple Reaction Monitoring (MRM)[1]
- Fluorescence microscopy[2]
- Step-wise photobleaching of dye-labelled subunits (photobleaching)[3]
- hydrophobic photolabeling (HyPLa)[4]
- peptide-based mass spectrometry[5]
- proteomics-based mass spectrometry[6]
- cell fusion and single molecule imaging[7]
- isotope-coded mass spectrometry[8]
--Wotheina (talk) 05:51, 8 August 2021 (UTC)
- 1 2 Schmidt, Carla; Lenz, Christof; Grote, Michael; LüHrmann, Reinhard; Urlaub, Henning (2010). "Determination of Protein Stoichiometry within Protein Complexes Using Absolute Quantification and Multiple Reaction Monitoring". Analytical Chemistry. 82 (7): 2784–2796. doi:10.1021/ac902710k. PMID 20225856.
- ↑ Geng, Jiefei; Klionsky, Daniel J. (2010). "Determining Atg protein stoichiometry at the phagophore assembly site by fluorescence microscopy". Autophagy. 6 (1): 144–147. doi:10.4161/auto.6.1.10249. PMC 2841983. PMID 20131413.
- ↑ Das, Somes K.; Darshi, Manjula; Cheley, Stephen; Wallace, Mark I.; Bayley, Hagan (2007). "Membrane Protein Stoichiometry Determined from the Step-Wise Photobleaching of Dye-Labelled Subunits". ChemBioChem. 8 (9): 994–999. doi:10.1002/cbic.200600474. PMID 17503420. S2CID 28971131.
- ↑ Giraldo, Ana María Villamil; Castello, Pablo Raúl; Flecha, F. Luis González; Moeller, Jesper V.; Delfino, José María; Rossi, Juan Pablo F.C. (2006). "Stoichiometry of lipid-protein interaction assessed by hydrophobic photolabeling". FEBS Letters. 580 (2): 607–612. doi:10.1016/j.febslet.2005.12.078. PMID 16412439. S2CID 43796110.
- ↑ Wohlgemuth, Ingo; Lenz, Christof; Urlaub, Henning (2015). "Studying macromolecular complex stoichiometries by peptide‐based mass spectrometry". Proteomics. 15 (5–6): 862–879. doi:10.1002/pmic.201400466. PMC 5024058. PMID 25546807.
- ↑ Smits, Arne H.; Jansen, Pascal W. T. C.; Poser, Ina; Hyman, Anthony A.; Vermeulen, Michiel (2013). "Stoichiometry of chromatin-associated protein complexes revealed by label-free quantitative mass spectrometry-based proteomics". Nucleic Acids Research. 41 (1): e28. doi:10.1093/nar/gks941. PMC 3592467. PMID 23066101.
- ↑ Singh, Avtar; Van Slyke, Alexander L.; Sirenko, Maria; Song, Alexander; Kammermeier, Paul J.; Zipfel, Warren R. (2020). "Stoichiometric analysis of protein complexes by cell fusion and single molecule imaging". Scientific Reports. 10 (1): 14866. doi:10.1038/s41598-020-71630-6. PMC 7483473. PMID 32913201.
- ↑ Hochleitner, Elisabeth O.; Kastner, Berthold; Fröhlich, Thomas; Schmidt, Alexander; Lührmann, Reinhard; Arnold, Georg; Lottspeich, Friedrich (2005). "Protein Stoichiometry of a Multiprotein Complex, the Human Spliceosomal U1 Small Nuclear Ribonucleoprotein". Journal of Biological Chemistry. 280 (4): 2536–2542. doi:10.1074/jbc.M409587200. PMID 15525645. S2CID 20396018.