Talk:Phred quality score

Untitled

Hi, please don't delete my page, I am new to this and am trying to create a tutorial page on how to use a Phred score- a molecular biology tool currently used by much of the academic community.

Tutorial pages fall under the "instruction manual" clause of WP:NOT. Wikibooks is a better place to put the material. ColourBurst 23:10, 22 February 2007 (UTC)

Good job girls!

That opening sentence needs serious rewriting. It should mention the subject in the first sentence. --UsaSatsui (talk) 06:17, 31 July 2008 (UTC)

This article would also benefit from distinguishing between NexGen and Sanger sequencing in the opening Paragraph -- fergycool 7 Jan 08 —Preceding unsigned comment added by Fergycool (talk • contribs) 10:32, 7 January 2009 (UTC)

Needs Basic Overhaul

I corrected some factual errors in the "history" section (gleaned from the documentation and commercial distributor), but it occurred to me that this article is really more about the program "Phred" itself, of which the quality scores are evidently only one aspect of. The article should probably be re-configured (and re-titled) appropriately – feel free to do so. Respectfully, Agricola44 (talk) 15:19, 10 October 2008 (UTC)

As part of an overhaul, should phred have an uppercase P? The original Ewing et al papers have it all lowercase, as does the above linked documentation.

Overhauled: split off description of Phred

I followed Agricola44's suggestion and split this article into two pages - one describing the program Phred titled Phred base calling, and one (this one) that just concentrates on quality scores. A lot of the original content was moved to Phred base calling. Added some historic information and relevant citations. Genomesequencer (talk) 16:49, 10 April 2009 (UTC)

Image

Base 79 of the image is called as G even though it's blatently A. Having worked with chromatograms all summer I find it hard to believe Phred would mess this one up... has the file definitely not been edited or anything? Evercat (talk) 19:48, 7 October 2011 (UTC)

What is an "sequencer trace?"

This seems to be a term that non-experts would not know about. It is used in the first paragraph. -DW — Preceding unsigned comment added by 75.151.110.58 (talk) 05:18, 31 January 2012 (UTC)

Broken external link

The link for "Long Reads with the KB Basecaller" appears to be broken. The Thermo Fisher server redirects to their home page. The following URL refers to a similar (but probably different) document that will probably fit your purpose here.

https://tools.thermofisher.com/content/sfs/brochures/cms_040500.pdf Csgehman (talk) 04:13, 15 August 2025 (UTC)

Symbols

There is a one-to-one correspondence between symbols and Phred quality scores: subtracting 33 from the symbol's codepoint gives the Phred quality score (at least from ! to Z). Alfa-ketosav (talk) 06:02, 17 August 2025 (UTC)