Talk:DNA extraction

Untitled

Much as a troublehooting guide is useful I do wonder whether or not this is appropriate for an encyclopedia, perhaps it could be labelled as common problems / solutions?

195.195.125.1 16:53, 26 February 2007 (UTC) J

I know that wikipedia is not the place to do this, but I have to find why is used sodium acetate in DNA extraction from filamentous fungi and I can´t find it. someone can help me, thanks :)--ometzit<col> 15:26, 1 March 2007 (UTC)

complaint

it is almost impossible to overstate how bad the detection segment is NO ONE does diphenylamine any more; that was obsolete years ago

Wiki-Assignment

Greetings fro all wikipedians ,

First of all, I would like to thank the author of this article for his/her work. I am a first year molecular biology master's student intending to edit this article as it was assigned to me as a coursework. I will participate as a student editor. Course name and code: (MLC501) Recent Developments in Biotechnology at Üsküdar University . Houda Abdelkader (talk) 14:42, 27 January 2023 (UTC)

"Isolasi DNA" listed at Redirects for discussion

The redirect Isolasi DNA has been listed at redirects for discussion to determine whether its use and function meets the redirect guidelines. Readers of this page are welcome to comment on this redirect at Wikipedia:Redirects for discussion/Log/2026 January 5 § Isolasi DNA until a consensus is reached. ArcticSeeress (talk) 09:44, 5 January 2026 (UTC)

Suggested addition: bead beating under the lysis step

Disclosure: I am associated with LabTIE International, a manufacturer of laboratory bead dispensing equipment (see my user page), so I am proposing this change here rather than editing the article directly. The proposed text cites only independent literature and does not mention or link my company.

The article currently notes that "mechanical or enzymatic methods" are often used for lysis, but gives no detail on mechanical lysis. I suggest adding something like the following to the lysis step:

"Mechanical lysis is commonly performed by bead beating, in which samples are shaken at high speed together with small abrasive beads (typically glass, ceramic, zirconium or steel), releasing DNA from cells that resist chemical or enzymatic lysis, such as mycobacteria, spores and plant tissue. In large-scale studies, extraction is commonly performed in 96-well plate format so that many samples can be processed in parallel, although this format requires precautions against well-to-well contamination."

Please feel free to reword, trim or reject as you see fit. Thank you. DivingGuru (talk) 20:24, 20 August 2026 (UTC)

 Not done: The {{request edit}} template is for requesting changes to semi-protected pages. For conflict of interest requests, please use {{Edit COI}} instead. In solidarity, Randomdude123459 | Talk | Contribs | 04:37, 21 August 2026 (UTC)

Thank you, and apologies for the wrong template. I have reactivated this as an {{Edit COI}} request. The proposed text and its two independent sources are unchanged; please reword, trim or decline as you see fit. DivingGuru (talk) 09:14, 21 August 2026 (UTC)

References