Rootletin

CROCC
Identifiers
Aliases	CROCC, ROLT, ciliary rootlet coiled-coil, rootletin, TAX1BP2
External IDs	OMIM: 615776; MGI: 3529431; HomoloGene: 16811; GeneCards: CROCC; OMA:CROCC - orthologs
Gene location (Human) Chr. Chromosome 1 (human) Band 1p36.13 Start 16,740,273 bp End 16,972,964 bp
Gene location (Mouse) Chr. Chromosome 4 (mouse) Band 4|4 D3 Start 140,743,948 bp End 140,787,861 bp
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in right uterine tube sural nerve right hemisphere of cerebellum olfactory zone of nasal mucosa granulocyte apex of heart right ovary canal of the cervix left uterine tube body of uterus Top expressed in neural layer of retina superior frontal gyrus lens olfactory tubercle retinal pigment epithelium ventricular zone otic vesicle primary visual cortex subiculum epithelium of lens More reference expression data BioGPS n/a
Gene ontology Molecular function structural molecule activity protein binding kinesin binding actin binding Cellular component centrosome photoreceptor inner segment plasma membrane ciliary rootlet actin cytoskeleton centriole extracellular exosome cytoskeleton cytoplasm cytosol microtubule organizing center cilium cell projection Biological process protein localization to organelle protein localization epithelial structure maintenance ciliary basal body organization establishment of organelle localization centriole-centriole cohesion cell projection organization cell cycle photoreceptor cell maintenance positive regulation of cilium assembly positive regulation of protein localization to cilium centrosome cycle Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 9696 230872 Ensembl ENSG00000058453 ENSMUSG00000040860 UniProt Q5TZA2 Q8CJ40 RefSeq (mRNA) NM_014675 NM_001145958 NM_172122 RefSeq (protein) NP_055490 NP_001139430 NP_742120 Location (UCSC) Chr 1: 16.74 – 16.97 Mb Chr 4: 140.74 – 140.79 Mb PubMed search
Wikidata
View/Edit Human View/Edit Mouse

Rootletin
Identifiers
Symbol	Rootletin
Pfam	PF15035
Available protein structures: PDB   PF15035 (ECOD; PDBsum)   AlphaFold PF15035

Rootletin, also known as ciliary rootlet coiled-coil protein (CROCC) is a protein in humans that is encoded by the CROCC gene. Rootletin is a component of the ciliary rootlet and, together with CEP68 and CEP250, is required for centrosome cohesion.

Rootletin is an important protein in the ciliary rootlet, particularly for the structure and is considered important protein in mitosis as a centrosome linker.

Function

This protein forms part of the ciliary rootlet structure. It also contributes to the centrosome cohesion before mitosis. Expression of rootletin leads to the formation of fibrous protein.

Structure

This protein is part of the structure of a ciliary rootlet. This cytoskeletal-like structure starts from the basal body at one end of the cilium and extends towards the nucleus. Its molecular structure consists of a globular head domain and a tail domain made up of coiled-coil structures.

Protein interactions

A large coiled-coil protein, C-Nap1, is a docking site for the fibrous tether to proximal ends of centrioles which Rootletin physically interacts with. Furthermore, Rootletin is phosphorylated by Nek2 kinase.

Further reading

• Ching YP, Chan SF, Jeang KT, Jin DY (2006). "The retroviral oncoprotein Tax targets the coiled-coil centrosomal protein TAX1BP2 to induce centrosome overduplication". Nat. Cell Biol. 8 (7): 717–24. doi:10.1038/ncb1432. hdl:10722/54244. PMID 16767081. S2CID 10140583.
• Gregory SG, Barlow KF, McLay KE, et al. (2006). "The DNA sequence and biological annotation of human chromosome 1". Nature. 441 (7091): 315–21. Bibcode:2006Natur.441..315G. doi:10.1038/nature04727. PMID 16710414.
• Andersen JS, Wilkinson CJ, Mayor T, et al. (2003). "Proteomic characterization of the human centrosome by protein correlation profiling". Nature. 426 (6966): 570–4. Bibcode:2003Natur.426..570A. doi:10.1038/nature02166. PMID 14654843. S2CID 4427303.
• Behrends U, Schneider I, Rössler S, et al. (2003). "Novel tumor antigens identified by autologous antibody screening of childhood medulloblastoma cDNA libraries". Int. J. Cancer. 106 (2): 244–51. doi:10.1002/ijc.11208. PMID 12800201.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. Bibcode:2002PNAS...9916899M. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Seki N, Ohira M, Nagase T, et al. (1998). "Characterization of cDNA clones in size-fractionated cDNA libraries from human brain". DNA Res. 4 (5): 345–9. doi:10.1093/dnares/4.5.345. PMID 9455484.