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pComb3H

From Wikipedia, the free encyclopedia

pComb3H, a derivative of pComb3 optimized for expression of human fragments, is a phagemid used to express proteins such as zinc finger proteins and antibody fragments on phage pili for the purpose of phage display selection.[1]

For the purpose of phage production, it contains the bacterial ampicillin resistance gene (for B-lactamase), allowing the growth of only transformed bacteria.[2]

References

[edit]
  1. "Addgene: PComb3HTT".
  2. Shen, Yongmei; Yang, Xiaochun; Dong, Ningzheng; Xie, Xiaofang; Bai, Xia; Shi, Yizhen (2007). "Generation and selection of immunized Fab phage display library against human B cell lymphoma". Cell Research. 17 (7): 650–660. doi:10.1038/cr.2007.57. ISSN 1001-0602.