KIAA0232
| KIAA0232 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Identifiers | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Aliases | KIAA0232 | |||||||||||||||||||||||||||||||||||||||||||||||||||||
| External IDs | MGI: 1261849; GeneCards: KIAA0232 | |||||||||||||||||||||||||||||||||||||||||||||||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Wikidata | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||
KIAA0232 is a nuclear phosphoserine protein which in humans is encoded by the KIAA0232 gene.
Gene
[edit]KIAA0232 is located at 4p16.1 neighboring TBC1 domain family member 14 and an uncharacterized locus. It has 10 exons which comprise its 4 known transcript variants.[5]
Expression
[edit]KIAA0232 is expressed fairly ubiquitously, but particularly highly in the brain relative to other tissues according to GEO normal tissue expression profiling.[6] Other notable areas of high expression identified by EST profiling include nerves, umbilical cord, and parathyroid.[7]
Homology
[edit]Paralogs
[edit]There are no known paralogs of KIAA0232.
Orthologs
[edit]KIAA0232 is conserved in most animals, including mammals, reptiles, birds, amphibians, insects, and as far back as Trichnella spiralis, a species of nematode. It is not found in fungi, plants, or prokaryotes.[8]
Protein
[edit]The KIAA0232 protein is 1395 amino acids in length with a molecular weight of 154.8kDa. It has higher than average frequencies of serine and glutamic acid residues as well as several multi-serine runs that are evolutionarily conserved. It has an isoelectric point of 4.52.[9][10]
Domains
[edit]KIAA0232 is largely composed of DUF4603.[11]
Subcellular localization
[edit]KIAA0232 is predicted to have nuclear localization.[9]
Post-translational modification
[edit]KIAA0232 is predicted by the tools at ExPASy to be highly phosphorylated, with 15 tyrosine specific sites, 25 threonine specific sites, and 103 serine specific sites.[12]
Interacting proteins
[edit]KIAA0232 is experimentally known to interact with YWHAZ,[13] DYRK1A, and DYRK1B.[14] via two hybrid interaction experiments. YWHAZ is an apoptotic path regulator known to bind to phosphoserine proteins. DYRK1A/B are dual-specificity tyrosine kinases. This corroborates the post-translational modification predictions.
References
[edit]- 1 2 3 GRCh38: Ensembl release 89: ENSG00000170871 – Ensembl, May 2017
- 1 2 3 GRCm38: Ensembl release 89: ENSMUSG00000029190 – Ensembl, May 2017
- ↑ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ↑ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ↑ "KIAA0232 KIAA0232 [Homo sapiens (human)] - Gene - NCBI". www.ncbi.nlm.nih.gov. Retrieved 2016-04-28.
- ↑ "NCBI GEO Expression Profiles".
- ↑ "EST Profile - Hs.79276". www.ncbi.nlm.nih.gov. Archived from the original on May 18, 2017. Retrieved 2016-05-09.
- ↑ "BLAST: Basic Local Alignment Search Tool". blast.ncbi.nlm.nih.gov. Retrieved 2016-05-09.
- 1 2 Workbench NB. "SAPS, Statistical Analysis of Protein Sequence". SDSC Biology Workbench.
- ↑ "PI, Isoelectric point determination". SDSC Biology Workbench. Archived from the original on 2003-08-11.
- ↑ Bank RP. "RCSB Protein Data Bank". Archived from the original on 2012-12-27.
- ↑ "NetPhos 2.0 Server". www.cbs.dtu.dk. Retrieved 2016-05-09.
- ↑ Bandyopadhyay S, Chiang CY, Srivastava J, Gersten M, White S, Bell R, et al. (October 2010). "A human MAP kinase interactome". Nature Methods. 7 (10): 801–805. doi:10.1038/nmeth.1506. PMC 2967489. PMID 20936779.
- ↑ Varjosalo M, Keskitalo S, Van Drogen A, Nurkkala H, Vichalkovski A, Aebersold R, et al. (April 2013). "The protein interaction landscape of the human CMGC kinase group". Cell Reports. 3 (4): 1306–1320. doi:10.1016/j.celrep.2013.03.027. hdl:20.500.11850/70524. PMID 23602568.