Thymidylate kinase
| thymidylate kinase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
 Thymidylate kinase dimer, Human | |||||||||
| Identifiers | |||||||||
| EC no. | 2.7.4.9 | ||||||||
| Databases | |||||||||
| BRENDA | enzyme data | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | enzyme entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| Rhea | reactions | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
| |||||||||
| Thymidylate kinase | |||||||
|---|---|---|---|---|---|---|---|
| Identifiers | |||||||
| Symbol | Thymidylate_kin | ||||||
| Pfam | PF02223 | ||||||
| InterPro | IPR000062 | ||||||
| PROSITE | PDOC01034 | ||||||
| |||||||
Thymidylate kinase (EC 2.7.4.9; dTMP kinase) catalyzes the phosphorylation of thymidine monophosphate (dTMP) to form thymidine diphosphate (dTDP) by transferring a phosphate group from the cofactor, adenosine triphosphate (ATP), which is converted to adenosine diphosphate (ADP):
The enzyme characterised from Escherichia coli and mouse hepatoma requires magnesium ion Mg2+.[1][2][3]
Thymidylate kinase is a ubiquitous enzyme of about 25 Kd and is important in the dTTP synthesis pathway for DNA synthesis. The function of dTMP kinase in eukaryotes comes from the study of a cell cycle mutant, cdc8, in Saccharomyces cerevisiae. Structural and functional analyses suggest that the cDNA codes for authentic human dTMP kinase. The mRNA levels and enzyme activities corresponded to cell cycle progression and cell growth stages.[4]
Thymidylate kinase's subfamily is predicted thymidylate kinase, TKRP1. InterPro: IPR014505
Human protein DTYMK contains this domain.
Structural studies
[edit]As of late 2007, 40 structures have been solved for this class of enzymes, with PDB accession codes PDB: 1E2D, PDB: 1E2E, PDB: 1E2F, PDB: 1E2G, PDB: 1E2Q, PDB: 1E98, PDB: 1E99, PDB: 1E9A, PDB: 1E9B, PDB: 1E9C, PDB: 1E9D, PDB: 1E9E, PDB: 1E9F, PDB: 1G3U, PDB: 1GSI, PDB: 1GTV, PDB: 1MRN, PDB: 1MRS, PDB: 1N5I, PDB: 1N5J, PDB: 1N5K, PDB: 1N5L, PDB: 1NMX, PDB: 1NMY, PDB: 1NMZ, PDB: 1NN0, PDB: 1NN1, PDB: 1NN3, PDB: 1NN5, PDB: 1TMK, PDB: 1W2G, PDB: 1W2H, PDB: 2CCG, PDB: 2CCJ, PDB: 2CCK, PDB: 2PBR, PDB: 2TMK, PDB: 3TMK, PDB: 4TMK, and PDB: 5TMP.
See also
[edit]References
[edit]- ↑ Hurwitz J (1959). "The enzymatic incorporation of ribonucleotides into polydeoxynucleotide material". J. Biol. Chem. 234 (9): 2351–2358. doi:10.1016/S0021-9258(18)69813-8. PMID 14405566.
- ↑ Nelson DJ, Carter CE (1969). "Purification and characterization of Thymidine 5-monophosphate kinase from Escherichia coli B". J. Biol. Chem. 244 (19): 5254–62. doi:10.1016/S0021-9258(18)63654-3. PMID 4899016.
- ↑ Kielley RK (1970). "Purification and properties of thymidine monophosphate kinase from mouse hepatoma". J. Biol. Chem. 245 (16): 4204–12. doi:10.1016/S0021-9258(18)62905-9. PMID 4323166.
- ↑ Li C, Huang SH, Tang A, Drisco B, Zhang SQ, Seeger R, Jong A (1994). "Human dTMP kinase: gene expression and enzymatic activity coinciding with cell cycle progression and cell growth". DNA Cell Biol. 13 (5): 461–471. doi:10.1089/dna.1994.13.461. PMID 8024690.
