Janus Green B

Janus Green B^[1]
Solid Janus Green B	Janus Green B Solution
Names
	IUPAC name 8-(4-Dimethylaminophenyl)diazenyl-N,N-diethyl-10-phenylphenazin-10-ium-2-amine chloride
	Other names Diazin Green S; Union Green B
Identifiers
CAS Number	2869-83-2 ;
3D model (JSmol)	Interactive image;
ChEBI	CHEBI:21184 ;
ChemSpider	68607 ;
ECHA InfoCard	100.018.814
EC Number	220-695-6;
PubChem CID	76123;
UNII	B9KQ101KHX ;
CompTox Dashboard (EPA)	DTXSID80883898 ;
	InChI InChI=1S/C30H31N6.ClH/c1-5-35(6-2)26-17-19-28-30(21-26)36(25-10-8-7-9-11-25)29-20-23(14-18-27(29)31-28)33-32-22-12-15-24(16-13-22)34(3)4;/h7-21H,5-6H2,1-4H3;1H/q+1;/p-1 Key: XXACTDWGHQXLGW-UHFFFAOYSA-M ;
	SMILES CCN(CC)C1=CC2=[N+](C3=C(C=CC(=C3)N=NC4=CC=C(C=C4)N(C)C)N=C2C=C1)C5=CC=CC=C5.[Cl-];
Properties
Chemical formula	C30H31ClN6
Molar mass	511.06 g/mol
	Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa). verify (what is ?) Infobox references

Janus Green B is a basic dye and vital stain used in histology. It is also used to stain mitochondria supravitally, as was introduced by Leonor Michaelis in 1900.^[2]

The indicator Janus Green B changes colour according to the amount of oxygen present.^[3] When oxygen is present, the indicator oxidizes to a blue colour. In the absence of oxygen, the indicator is reduced and changes to a pink colour.

References

↑ Janus Green B, at Sigma-Aldrich
↑ Michaelis, L. (1900). Die vitale Farbung, eine Darstellungsmethode der Zellgranula. Archiv für mikroskopische Anatomie 55:558-575(Also Tafel XXXII)
↑ "Archived copy". Archived from the original on 2006-02-24. Retrieved 2007-10-31.{{cite web}}: CS1 maint: archived copy as title (link)

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[1] Janus Green B, at Sigma-Aldrich

[2] Michaelis, L. (1900). Die vitale Farbung, eine Darstellungsmethode der Zellgranula. Archiv für mikroskopische Anatomie 55:558-575(Also Tafel XXXII)

[3] "Archived copy". Archived from the original on 2006-02-24. Retrieved 2007-10-31.{{cite web}}: CS1 maint: archived copy as title (link)

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v t e Microbial and histological stains
Iron/hemosiderin	Perls Prussian blue
Lipids	Sudan stain Sudan II Sudan III Sudan IV Oil Red O Sudan Black B
Carbohydrates	Alcian blue Mucicarmine Periodic acid–Schiff stain
Amyloid	Congo red Thioflavin
Bacteria	Gram stain Methyl violet/Gentian violet Safranin Acid-fast Ziehl–Neelsen stain/Kinyoun stain Carbol fuchsin/Fuchsine Methylene blue Auramine–rhodamine stain Auramine O Rhodamine B Auramine phenol stain
Connective tissue	trichrome stain: Masson's trichrome stain/Lillie's trichrome Light Green SF yellowish Biebrich scarlet Phosphomolybdic acid Fast Green FCF Sirius Red Van Gieson's stain
Other	Cresyl violet Cyanine Jaswant Singh–Bhattacharji (JSB) stain H&E stain Haematoxylin Eosin Y Janus Green B Giemsa stain Gömöri trichrome stain Luxol fast blue stain Methyl blue Moeller stain Movat's stain Neutral red Schaeffer–Fulton stain Silver stain Bielschowsky stain Grocott's methenamine silver stain Warthin–Starry stain Wright's stain
Tissue stainability	Acidophilic Basophilic Chromophobic

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References