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Inner centromere protein

From Wikipedia, the free encyclopedia
(Redirected from INCENP)

INCENP
Identifiers
AliasesINCENP, inner centromere protein
External IDsOMIM: 604411; MGI: 1313288; GeneCards: INCENP
Available structures
PDBOrtholog search: PDBe RCSB
Orthologs
DatabasesNCBI: entry; OMA: entry
SpeciesHumanMouse
Entrez

3619

16319

Ensembl

ENSG00000149503

ENSMUSG00000024660

UniProt

Q9NQS7

Q9WU62

RefSeq (mRNA)

NM_001040694
NM_020238

NM_016692
NM_001369356

RefSeq (protein)

NP_001035784
NP_064623

NP_057901

Location (UCSC)Chr 11: 62.12 – 62.15 MbChr 19: 9.85 – 9.88 Mb
PubMed search[3][4]
Wikidata
View/Edit HumanView/Edit Mouse
Chromosome passenger complex (CPC) protein INCENP N terminal
Identifiers
SymbolINCENP_N
PfamPF12178
InterProIPR022006
Available protein structures:
PDB  IPR022006 PF12178 (ECOD; PDBsum)  
AlphaFold
Inner centromere protein, ARK binding region
Identifiers
SymbolINCENP_ARK-bind
PfamPF03941
InterProIPR005635
Available protein structures:
PDB  IPR005635 PF03941 (ECOD; PDBsum)  
AlphaFold

Inner centromere protein is a protein that in humans is encoded by the INCENP gene.[5][6][7] It is a regulatory protein in the chromosome passenger complex (CPC).[8] It is involved in regulation of the catalytic proteins Aurora B and Aurora C.[9][10][11] It acts in association with two other proteins - Survivin and Borealin. These proteins form a tight three-helical bundle. The N-terminal domain of INCENP is the domain involved in formation of this three-helical bundle while its C-terminal domain is responsible for the interaction with Aurora B.[12][11]

In mammalian cells, two broad groups of centromere-interacting proteins have been described: constitutively binding centromere proteins and 'passenger' (or transiently interacting) proteins.[13] The constitutive proteins include CENPA (centromere protein A), CENPB, CENPC1, and CENPD.

The term 'passenger proteins' encompasses a broad collection of proteins that localize to the centromere during specific stages of the cell cycle.[14] These include CENPE; MCAK; KID; cytoplasmic dynein (e.g., DYNC1H1); CliPs (e.g. CLIP1); and CENPF/mitosin (CENPF). The inner centromere proteins (INCENPs),[5] the initial members of the passenger protein group, display a broad localization along chromosomes in the early stages of mitosis but gradually become concentrated at centromeres as the cell cycle progresses into mid-metaphase. During telophase, the proteins are located within the midbody in the intercellular bridge, where they are discarded after cytokinesis.[7][15]

Interactions

[edit]

INCENP has been shown to interact with H2AFZ,[16] Survivin[17] and CDCA8.[18] The ARK binding region has been found to be necessary and sufficient for binding to aurora-related kinase. This interaction has been implicated in the coordination of chromosome segregation with cell division in yeast.[19]

References

[edit]
  1. 1 2 3 GRCh38: Ensembl release 89: ENSG00000149503 – Ensembl, May 2017
  2. 1 2 3 GRCm38: Ensembl release 89: ENSMUSG00000024660 – Ensembl, May 2017
  3. ↑ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  4. ↑ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  5. 1 2 Earnshaw WC, Cooke CA (September 1991). "Analysis of the distribution of the INCENPs throughout mitosis reveals the existence of a pathway of structural changes in the chromosomes during metaphase and early events in cleavage furrow formation". Journal of Cell Science. 98 (4): 443–461. doi:10.1242/jcs.98.4.443. PMID 1860899.
  6. ↑ Adams RR, Eckley DM, Vagnarelli P, Wheatley SP, Gerloff DL, Mackay AM, et al. (July 2001). "Human INCENP colocalizes with the Aurora-B/AIRK2 kinase on chromosomes and is overexpressed in tumour cells". Chromosoma. 110 (2): 65–74. doi:10.1007/s004120100130. PMID 11453556. S2CID 13778759.
  7. 1 2 "Entrez Gene: INCENP inner centromere protein antigens 135/155kDa".
  8. ↑ Dietz A, Subedi P, Azimzadeh O, Duchrow L, Kaestle F, Paetzold J, et al. (2024). "The Chromosome Passenger Complex (CPC) Components and Its Associated Pathways Are Promising Candidates to Differentiate Between Normosensitive and Radiosensitive ATM-Mutated Cells". Biomarker Insights. 19 11772719241274017. doi:10.1177/11772719241274017. PMC 11528597. PMID 39493730.
  9. ↑ Sasai K, Katayama H, Hawke DH, Sen S (2016). "Aurora-C Interactions with Survivin and INCENP Reveal Shared and Distinct Features Compared with Aurora-B Chromosome Passenger Protein Complex". PLOS ONE. 11 (6) e0157305. Bibcode:2016PLoSO..1157305S. doi:10.1371/journal.pone.0157305. PMC 4917241. PMID 27332895.
  10. ↑ Li X, Sakashita G, Matsuzaki H, Sugimoto K, Kimura K, Hanaoka F, et al. (November 2004). "Direct association with inner centromere protein (INCENP) activates the novel chromosomal passenger protein, Aurora-C". The Journal of Biological Chemistry. 279 (45): 47201–47211. doi:10.1074/jbc.m403029200. PMID 15316025.
  11. 1 2 Honda R, Körner R, Nigg EA (August 2003). "Exploring the functional interactions between Aurora B, INCENP, and survivin in mitosis". Molecular Biology of the Cell. 14 (8): 3325–3341. doi:10.1091/mbc.e02-11-0769. PMC 181570. PMID 12925766.
  12. ↑ Jeyaprakash AA, Klein UR, Lindner D, Ebert J, Nigg EA, Conti E (October 2007). "Structure of a Survivin-Borealin-INCENP core complex reveals how chromosomal passengers travel together". Cell. 131 (2): 271–285. doi:10.1016/j.cell.2007.07.045. PMID 17956729. S2CID 15199822.
  13. ↑ Choo, K. H. Andy (1997). The centromere. Oxford [Oxfordshire]: Oxford University Press. ISBN 978-0-19-857780-5.
  14. ↑ Earnshaw WC, Mackay AM (September 1994). "Role of nonhistone proteins in the chromosomal events of mitosis". FASEB Journal. 8 (12): 947–956. doi:10.1096/fasebj.8.12.8088460. PMID 8088460. S2CID 14062246.
  15. ↑ Cutts SM, Fowler KJ, Kile BT, Hii LL, O'Dowd RA, Hudson DF, et al. (July 1999). "Defective chromosome segregation, microtubule bundling and nuclear bridging in inner centromere protein gene (Incenp)-disrupted mice". Human Molecular Genetics. 8 (7): 1145–1155. doi:10.1093/hmg/8.7.1145. PMID 10369859.
  16. ↑ Rangasamy D, Berven L, Ridgway P, Tremethick DJ (April 2003). "Pericentric heterochromatin becomes enriched with H2A.Z during early mammalian development". The EMBO Journal. 22 (7): 1599–1607. doi:10.1093/emboj/cdg160. PMC 152904. PMID 12660166.
  17. ↑ Wheatley SP, Carvalho A, Vagnarelli P, Earnshaw WC (June 2001). "INCENP is required for proper targeting of Survivin to the centromeres and the anaphase spindle during mitosis". Current Biology. 11 (11): 886–890. Bibcode:2001CBio...11..886W. doi:10.1016/S0960-9822(01)00238-X. PMID 11516652.
  18. ↑ Gassmann R, Carvalho A, Henzing AJ, Ruchaud S, Hudson DF, Honda R, et al. (July 2004). "Borealin: a novel chromosomal passenger required for stability of the bipolar mitotic spindle". The Journal of Cell Biology. 166 (2): 179–191. doi:10.1083/jcb.200404001. PMC 2172304. PMID 15249581.
  19. ↑ Leverson JD, Huang HK, Forsburg SL, Hunter T (April 2002). "The Schizosaccharomyces pombe aurora-related kinase Ark1 interacts with the inner centromere protein Pic1 and mediates chromosome segregation and cytokinesis". Molecular Biology of the Cell. 13 (4): 1132–1143. doi:10.1091/mbc.01-07-0330. PMC 102257. PMID 11950927.

Further reading

[edit]
This article incorporates text from the public domain Pfam and InterPro: IPR005635
This article incorporates text from the public domain Pfam and InterPro: IPR022006
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