4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase
| 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 1.17.7.1 | ||||||||
| Databases | |||||||||
| BRENDA | enzyme data | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | enzyme entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| Rhea | reactions | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
| |||||||||
4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (HMB-PP synthase, IspG, EC 1.17.7.1) is an enzyme that catalyzes a redox reaction
The substrate of this enzyme is 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MEcPP) and the product is (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate (HMB-PP). Electrons are donated by two reduced ferredoxin proteins per reaction.[1] The enzyme characterised from Thermosynechococcus elongatus contains an iron-sulfur cluster.[2][3][4]
The reaction is part of the non-mevalonate pathway (MEP pathway) of isoprenoid precursor biosynthesis.[5]
Nomenclature
[edit]This enzyme is an oxidoreductase, specifically one acting on CH or CH2 groups with a disulfide as acceptor. The systematic name of this enzyme class is (E)-4-hydroxy-3-methylbut-2-en-1-yl-diphosphate:protein-disulfide oxidoreductase (hydrating).[6]
References
[edit]- ↑ Hecht, Stefan; Eisenreich, Wolfgang; Adam, Petra; Amslinger, Sabine; Kis, Klaus; Bacher, Adelbert; Arigoni, Duilio; Rohdich, Felix (2001). "Studies on the nonmevalonate pathway to terpenes: The role of the GcpE (IspG) protein". Proceedings of the National Academy of Sciences. 98 (26): 14837–14842. doi:10.1073/pnas.201399298. PMC 64945. PMID 11752431.
- ↑ Okada, Ken; Hase, Toshiharu (2005). "Cyanobacterial Non-mevalonate Pathway". Journal of Biological Chemistry. 280 (21): 20672–20679. doi:10.1074/jbc.M500865200. PMID 15792953.
- ↑ Seemann, Myriam; Wegner, Patrick; Schunemann, Volker; Bui, Bernadette Tse Sum; Wolff, Murielle; Marquet, Andrée; Trautwein, Alfred X.; Rohmer, Michel (2005). "Isoprenoid biosynthesis in chloroplasts via the methylerythritol phosphate pathway: The (E)-4-hydroxy-3-methylbut-2-enyl diphosphate synthase (GcpE) from Arabidopsis thaliana is a [4Fe?4S] protein". Journal of Biological Inorganic Chemistry. 10 (2): 131–137. doi:10.1007/s00775-004-0619-z. PMID 15650872.
- ↑ Seemann, Myriam; Bui, Bernadette Tse Sum; Wolff, Murielle; Tritsch, Denis; Campos, Narciso; Boronat, Albert; Marquet, Andrée; Rohmer, Michel (2002). "Isoprenoid Biosynthesis through the Methylerythritol Phosphate Pathway: The (E)-4-Hydroxy-3-methylbut-2-enyl Diphosphate Synthase (GcpE) is a [4Fe–4S] Protein". Angewandte Chemie International Edition. 41 (22): 4337–4339. doi:10.1002/1521-3773(20021115)41:22<4337::AID-ANIE4337>3.0.CO;2-K. PMID 12434382.
- ↑ Seemann, Myriam; Tse Sum Bui, Bernadette; Wolff, Murielle; Miginiac-Maslow, Myroslawa; Rohmer, Michel (2006). "Isoprenoid biosynthesis in plant chloroplasts via the MEP pathway: Direct thylakoid/Ferredoxin-dependent photoreduction of GcpE/IspG". FEBS Letters. 580 (6): 1547–1552. doi:10.1016/j.febslet.2006.01.082. PMID 16480720.
- ↑ Enzyme 1.17.7.1 at KEGG Pathway Database.
