Draft:Russell Higuchi
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Submission declined on 6 March 2026 by ChrysGalley (talk).
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This draft is not adequately supported by reliable sources. Wikipedia's verifiability policy requires that all content be supported by reliable sources.
Declined by Rambley 8 months ago.
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Comment: The sources are not backing the text and show signs of LLM suggestive thinking. So for example source 6 makes 2 passing references to Higuchi (carefully noting he was one of many) and does not use the word pioneering. Source 5 is an 800 pages book and yet no page number is provided. ChrysGalley (talk) 23:11, 6 March 2026 (UTC)
- Thank you for the detailed review and for pointing out the issues.
- I understand the concerns regarding the wording and the alignment between the text and the cited sources. I will revise the draft to ensure that all statements closely follow the wording and scope of the sources. In particular, I will remove evaluative terms that are not explicitly used in the references and will add page numbers for book sources such as *A-Z of Quantitative PCR*.
- Thank you again for the helpful feedback. I will update the draft accordingly. De La Rue Thomas (talk) 03:30, 7 March 2026 (UTC)
Russell G. Higuchi is a molecular biologist known for research on the development of real-time polymerase chain reaction (real-time PCR).[1] In 2019, the Association for Molecular Pathology presented him with its Award for Excellence in Molecular Diagnostics.[2]
In 1992, Higuchi and his colleagues reported a method that allowed PCR amplification to be detected without opening the reaction tube. They added ethidium bromide to the reaction mixture and monitored the increase in fluorescence as double-stranded DNA accumulated.[3][4][5]
The following year, Higuchi and his colleagues described a quantitative assay that used a video camera to monitor several PCRs during thermocycling. The fluorescence curves reflected the accumulation of double-stranded DNA and were related to the amount of DNA present at the start of the reaction.[6]
Subsequent reviews have described the 1992–1993 work of Higuchi and his colleagues in the development of real-time PCR.[1][4][5] In a 2013 retrospective on the history of PCR, Mikael Kubista described how Higuchi's group used changes in fluorescence to follow amplification during the reaction.[7] A 2015 review in Clinica Chimica Acta described Higuchi and his colleagues as having pioneered the analysis of PCR kinetics.[8]
References
[edit]- 1 2 Kubista, Mikael; Andrade, José Manuel; Bengtsson, Martin; Forootan, Amin; Jonák, Jiří; Lind, Kristina; Sindelka, Radek; Sjöback, Robert; Sjögreen, Björn; Strömbom, Linda; Ståhlberg, Anders; Zoric, Neven (2006). "The real-time polymerase chain reaction". Molecular Aspects of Medicine. 27 (2–3): 95–125. doi:10.1016/j.mam.2005.12.007. PMID 16460794.
- ↑ "Association for Molecular Pathology Announces 2019 Award Recipients". Association for Molecular Pathology. 19 September 2019. Retrieved 28 July 2026 – via Newswise.
- ↑ Higuchi, Russell; Dollinger, Gavin; Walsh, P. Sean; Griffith, Robert (1992). "Simultaneous amplification and detection of specific DNA sequences". Bio/Technology. 10 (4): 413–417. doi:10.1038/nbt0492-413. PMID 1368485.
- 1 2 Kaltenboeck, Bernhard; Wang, Chengming (2005). "Advances in Real-Time PCR: Application to Clinical Laboratory Diagnostics". Advances in Clinical Chemistry. 40: 219–259. doi:10.1016/S0065-2423(05)40006-2. PMC 7112074. PMID 16355924.
- 1 2 Monis, Paul T.; Giglio, Steven (2006). "Nucleic acid amplification-based techniques for pathogen detection and identification". Infection, Genetics and Evolution. 6 (1): 2–12. doi:10.1016/j.meegid.2005.08.004. PMC 7106022. PMID 16169776.
- ↑ Higuchi, Russell; Fockler, Carita; Dollinger, Gavin; Watson, Robert (1993). "Kinetic PCR analysis: Real-time monitoring of DNA amplification reactions". Bio/Technology. 11 (9): 1026–1030. doi:10.1038/nbt0993-1026. PMID 7764001.
- ↑ Kubista, Mikael (1 November 2013). "PCR @ 30: 30 Years Young and Still Evolving". Genetic Engineering & Biotechnology News. Retrieved 28 July 2026.
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