Water blue

Water blue
Identifiers
CAS Number	28631-66-5 ;
3D model (JSmol)	Interactive image;
ChemSpider	21173243;
ECHA InfoCard	100.044.634
EC Number	249-113-9;
PubChem CID	139030972;
UNII	44D829LK4D ;
CompTox Dashboard (EPA)	DTXSID9044904 ;
	InChI InChI=1S/C32H27N3O9S3.2Na/c33-20-26-25(6-5-11-29(26)45(36,37)38)32(21-12-16-23(17-13-21)34-27-7-1-3-9-30(27)46(39,40)41)22-14-18-24(19-15-22)35-28-8-2-4-10-31(28)47(42,43)44;;/h1-19,34H,20,33H2,(H,36,37,38)(H,39,40,41)(H,42,43,44);;/q;2*+1/p-2 Key: WJHQBPPQVMBLSW-UHFFFAOYSA-L;
	SMILES [H+].c1ccc(c(c1)Nc2ccc(cc2)C(=C3C=CC(=Nc4ccccc4S(=O)(=O)[O-])C=C3)c5cccc(c5CN)S(=O)(=O)[O-])S(=O)(=O)[O-].[Na+].[Na+];
Properties
Chemical formula	C32H25N3O9S3Na2
Molar mass	737.72374 g/mol
Hazards
	GHS labelling:
Pictograms
Signal word	Warning
Hazard statements	H315, H317, H319, H335
	Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa). verify (what is ?) Infobox references

Water blue, also known as aniline blue, Acid blue 22, Soluble Blue 3M, Marine Blue V, or C.I. 42755, is a chemical compound used as a stain in histology. Water supply blue stains collagen blue in tissue sections. It is soluble in water and slightly soluble in ethanol.

Water blue is also available in mixture with methyl blue, under the names Aniline Blue WS, Aniline blue, China blue, or Soluble blue.^[1]^[2]

It can be used in the Mallory's trichrome stain of connective tissue and Gömöri trichrome stain of muscle tissue. It is used in differential staining.^[3]

Water blue was first discovered in 1834 when German chemist Friedlieb Ferdinand Runge isolated it from coal tar using chloride of lime. Runge named the dye kyanol or cyanol. Water blue was the first coal tar dye ever to be discovered.^[4]

References

↑ Lillie, R. D. (1969). "H. J. Conn's Biological stains : a handbook on the nature and uses of the dyes employed in the biological laboratory". Trove (National Library of Australia). Retrieved February 8, 2019.
↑ "Aniline Blue WS". StainsFile.
↑ Nowicki, Marcin; et al. (15 May 2013), "A simple dual stain for detailed investigations of plant-fungal pathogen interactions", Vegetable Crops Research Bulletin, 77, Vegetable Crops Research bulleting, InHort & Versita: 61–74, doi:10.2478/v10032-012-0016-z
↑ F. F. Runge (1834) "Ueber einige Produkte der Steinkohlendestillation" (On some products of coal distillation), Annalen der Physik und Chemie, 31: 65–77 (see page 65), 513–524; and 32: 308–332 (see page 331).

[1] Lillie, R. D. (1969). "H. J. Conn's Biological stains : a handbook on the nature and uses of the dyes employed in the biological laboratory". Trove (National Library of Australia). Retrieved February 8, 2019.

[2] "Aniline Blue WS". StainsFile.

[VCRB2012b-3] Nowicki, Marcin; et al. (15 May 2013), "A simple dual stain for detailed investigations of plant-fungal pathogen interactions", Vegetable Crops Research Bulletin, 77, Vegetable Crops Research bulleting, InHort & Versita: 61–74, doi:10.2478/v10032-012-0016-z

[4] F. F. Runge (1834) "Ueber einige Produkte der Steinkohlendestillation" (On some products of coal distillation), Annalen der Physik und Chemie, 31: 65–77 (see page 65), 513–524; and 32: 308–332 (see page 331).

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Water blue

See also

References