Talk:Daisy chaining DNA
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[edit]I was thinking of editing this stub to use simpler language and explain exactly what a daisy chain of DNA is. The change I want to make is for the article to say this: "Daisy chaining DNA is the process of when DNA undergoing PCR becomes tangled and forms a 'daisy chain.' During PCR, primers or dNTP's will eventually be used up and limit further reactions; it is when the primers deplete that causes daisy chaining. Since the denaturing and annealing processes still continue without primers, the single-stranded DNA molecules reanneal to themselves. However, this reannealing does not always occur to another complementary strand. It is this imperfect match up that causes 'tangles'. These tangles look like a daisy chain." I used this source, and will of course site it. [1] Thoughts? (Kathryn.cc (talk) 02:20, 23 September 2015 (UTC))
References
- ↑ "High-Throughput NGS Library Preparation Technical Guide". Kapa Biosystems. Retrieved 23 September 2015.
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Wiki Education Foundation-supported course assignment
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This article was the subject of a Wiki Education Foundation-supported course assignment, between 24 August 2020 and 11 December 2020. Further details are available on the course page. Peer reviewers: Cmbio401.
Above undated message substituted from Template:Dashboard.wikiedu.org assignment by PrimeBOT (talk) 13:03, 18 January 2022 (UTC)
Daisy chain gene drives are not related to Daisy chaining in PCR.
[edit]I suggest removing the text pertaining to gene drives. It was incorrectly added to a completely different topic (daisy chaining of PCR products) and the text is of low quality or just plain wrong. At the very least they should be split into two separate pages. I am less familiar with the PCR-based phenomena, but I am very familiar with the gene drive one. I don't think daisy chain gene drives merit their own page when the broader category of self-limiting/localised gene drives does not have a page. Examples of other types of self-limiting designs can be found here (many of those have actually been experimentally demonstrated in contrast to daisy-chain drives): https://www.pnas.org/doi/10.1073/pnas.2020417117 OrganicCoder (talk) 15:13, 8 February 2023 (UTC)
- Completely agree - this text is complete nonsense. PCR daisy chaining has absolutely nothing to do with gene editing. It can happen during PCR as a result of primer exhaustion - that much is true - but that's about it. There is no connection to gene drive or CRISPR. ~2026-22034-34 (talk) 07:25, 10 April 2026 (UTC)