GABA transporter type 2
| SLC6A13 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
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| Aliases | SLC6A13, GAT-2, GAT2, solute carrier family 6 member 13, GABA transporter type 2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||
| External IDs | OMIM: 615097; MGI: 95629; GeneCards: SLC6A13 | |||||||||||||||||||||||||||||||||||||||||||||||||||||
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| Wikidata | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
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GABA transporter 2 (GAT2; SLC6A13) also known as sodium- and chloride-dependent GABA transporter 2 is one of four GABA transporters, GAT1 (SLC6A1), GAT2 (SLC6A13), GAT3 (SLC6A11) and BGT1 (SLC6A12).[5] Note that GAT2 is different from BGT1 despite the fact that the latter transporter is sometimes referred at as (mouse) GAT-2.
All these transporters are highly hydrophobic proteins with 12 transmembrane segments, extracellular glycosylation sites, and intracellular consensus sites for phosphorylation, and there is over 50% amino acid homology between each of them. Each binds GABA with varying affinities with BGT1 having the lowest affinity and GAT3 the highest. GAT2 (SLC6A13) is predominantly expressed in hepatocytes in the liver, but is also found in proximal tubules in the kidney as well as in the leptomeninges and in some blood vessels in the brain.[6]
Biological function
[edit]Brain
[edit]Deletion of the GAT2 gene in mice does not appear to have any dramatic effects on brain function in a normal situation. The only difference noted so far is a slight elevation of brain Taurine levels.[6] This was an unexpected finding, but is in agreement with the notion that GAT2 permits efflux of GABA and taurine from the brain to circulating blood through the blood brain barrier.[7] GAT1 and GAT3 have higher concentrations in the brain and have higher affinity to GABA. This makes them more likely than GAT2 to influence the activity of neurotransmitter GABA in the brain.[5]
Liver
[edit]GAT2 is expressed in hepatocytes and is well positioned to take up GABA entering the liver from the intestine (via the portal vein), but it is not known if this is an important function. On the other hand, GAT2 is also able to transport taurine and it appears to be the major taurine transporter in the liver.[6]
Kidneys
[edit]GAT2 is also present in proximal tubules in the kidney cortex, but only in the basolateral membranes. The physiological function is unknown.[6]
Clinical significance
[edit]The clinical significance of GAT2 is at present undetermined.
Some anticonvulsants have been reported to act upon the GABA transporters. There is evidence to suggest that GABA transporters are linked to epilepsy, affective disorders, and schizophrenia. But considering that GAT1 and GAT3 are expressed at far higher levels in brain tissue, it is likely that inhibition of these transporters will have a far greater anticonvulsant effect than inhibition of GAT2[6] and BGT1.[8]
References
[edit]- 1 2 3 GRCh38: Ensembl release 89: ENSG00000010379 – Ensembl, May 2017
- 1 2 3 GRCm38: Ensembl release 89: ENSMUSG00000030108 – Ensembl, May 2017
- ↑ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ↑ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- 1 2 Zhou Y, Danbolt NC (2013). "GABA and Glutamate Transporters in Brain". Frontiers in Endocrinology. 4 165. Lausanne. doi:10.3389/fendo.2013.00165. PMC 3822327. PMID 24273530.
- 1 2 3 4 5 Zhou Y, Holmseth S, Guo C, Hassel B, Höfner G, Huitfeldt HS, et al. (2012). "Deletion of the γ-aminobutyric acid transporter 2 (GAT2 and SLC6A13) gene in mice leads to changes in liver and brain taurine contents". The Journal of Biological Chemistry. 287 (42): 35733–35746. doi:10.1074/jbc.M112.368175. PMC 3471754. PMID 22896705.
- ↑ Takanaga H, Ohtsuki S, Hosoya K, Terasaki T (2001). "GAT2/BGT-1 as a system responsible for the transport of gamma-aminobutyric acid at the mouse blood-brain barrier". Journal of Cerebral Blood Flow and Metabolism. 21 (10): 1232–1239. doi:10.1097/00004647-200110000-00012. PMID 11598501.
- ↑ Lehre AC, Rowley NM, Zhou Y, Holmseth S, Guo C, Holen T, et al. (2011). "Deletion of the betaine-GABA transporter (BGT1; slc6a12) gene does not affect seizure thresholds of adult mice". Epilepsy Research. 95 (1–2): 70–81. doi:10.1016/j.eplepsyres.2011.02.014. PMC 3376448. PMID 21459558.
Further reading
[edit]- Minelli A, Brecha NC, Karschin C, DeBiasi S, Conti F (November 1995). "GAT-1, a high-affinity GABA plasma membrane transporter, is localized to neurons and astroglia in the cerebral cortex". The Journal of neuroscience : the official journal of the Society for Neuroscience. 15 (11): 7734–46. doi:10.1523/JNEUROSCI.15-11-07734.1995. PMC 6578038. PMID 7472524.
- Ikegaki N, Saito N, Hashima M, Tanaka C (October 1994). "Production of specific antibodies against GABA transporter subtypes (GAT1, GAT2, GAT3) and their application to immunocytochemistry". Brain Research. Molecular Brain Research. 26 (1–2): 47–54. doi:10.1016/0169-328x(94)90072-8. hdl:20.500.14094/D1001458. PMID 7854065.