J558L cells
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J558L cells are mouse B myeloma cells derived from a BALB/c strain isolated by M. Bruggeman and M.S. Neuberger in the University of Cambridge.[1][2] It is a stable B cell line used to express antibodies. They are a suspension cell line and are best cultured in Eagle's minimal essential medium with 10% Fetal bovine serum, 0.01 units/ml of penicillin and 0.1 mg/ml streptomycin incubated in 37 °C + 5% CO2 + 90% relative humidity with a cell density of 5 × 105 cell per 15 ml.
Uses
[edit]This cell line is usually used to express antibodies since they can result in antibody titers up to 1 mg under optimum conditions.[citation needed]
References
[edit]- ↑ Oi, VT; Morrison, SL; Herzenberg, LA; Berg, P (1983). "Immunoglobulin gene expression in transformed lymphoid cells". Proc. Natl. Acad. Sci. U.S.A. 80 (3): 825–9. Bibcode:1983PNAS...80..825O. doi:10.1073/pnas.80.3.825. PMC 393473. PMID 6402777.
- ↑ "Detail".