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Endo agar (also called Endo's medium) is a microbiological growth medium with a faint pink colour.[1] Originally developed for the isolation of Salmonella typhi, it is now used mostly as a coliform medium. Most gram-negative organisms grow well in this medium, while growth of gram-positive organisms is inhibited.[2] Coliform organisms ferment the lactose in this medium, producing a green metallic sheen (i.e. Escherichia coli), whereas non-lactose-fermenting organisms produce clear, colourless colonies,[1] i.e. Salmonella species.
Typical composition
[edit]Endo agar typically contains (w/v):[3]
- 1.0 % peptone
- 0.25 % dipotassium hydrogen phosphate (K2HPO4)
- 1.0 % lactose
- 0.33 % anhydrous sodium sulfite (Na2SO3)
- 0.03 % fuchsine
- 1.25 % agar
References
[edit]- 1 2 "Mondofacto". Archived from the original on March 3, 2016. Retrieved April 25, 2009.
- ↑ "Traditional Microbiology". Becton, Dickinson and Co. (BD). 2006. Archived from the original on March 3, 2016. Retrieved November 28, 2022.
- ↑ EMD Chemicals, "ENDO Agar". 2002. Archived from the original on April 13, 2009. Retrieved April 25, 2009.
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