Draft:N2G mouse
Submission declined on 30 July 2026 by Wikiman2230 (talk).
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Submission declined on 25 September 2025 by Shocksingularity (talk). This draft is not written from a neutral point of view. Wikipedia articles must be written neutrally in a formal, impersonal, and dispassionate way. They should not read like a blog post, advertisement, or fan page. Rewrite the draft to remove:
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Comment: Please re-write this in a neutral tone. And also maybe get more citations while your at it. Wikiman (Talk) 21:44, 30 July 2026 (UTC)
- Comment: Prose and possibly-hallucinated infobox is making me sus ai Shocksingularity (talk) 04:37, 25 September 2025 (UTC)
The N2G mouse (NOD–2–Genes knockout) is a severely immunodeficient laboratory mouse strain developed on a NOD background. The strain was generated by GEMCRO Inc. (South Korea) using CRISPR/Cas9 to delete large genomic regions of Prkdc, which encodes DNA-dependent protein kinase catalytic subunit (DNA-PKcs), and Il2rg, which encodes the interleukin-2 receptor common gamma chain (γc).[1]
Genetics and immunophenotype
[edit]Loss of Prkdc function disrupts V(D)J recombination, resulting in the absence of mature T and B lymphocytes. Deletion of Il2rg additionally prevents natural killer cell development. The combined deficiency produces a T−B−NK− phenotype comparable to that of the NSG mouse and NOG mouse strains, which carry loss-of-function mutations in the same two genes.[1][2][3]
Applications
[edit]Human cancer xenografts
[edit]N2G mice support subcutaneous engraftment of human cancer cell lines and have been used to evaluate targeted therapies in vivo. The strain has been employed to investigate aurora kinase B inhibition as a strategy to overcome resistance to MET-targeted drugs in MET-amplified lung cancer models.[4] In a separate study, N2G mice were used to examine the role of dysfunctional adipocytes in tumor progression through YAP/TAZ-dependent cancer-associated adipocyte transformation.[5]
Hematopoietic stem cell engraftment
[edit]The strain supports engraftment of human hematopoietic stem cells (HSCs) and subsequent reconstitution of human immune cell populations.[1]
Cell therapy evaluation
[edit]A derivative strain, N2G-F8, carries an additional deletion of the F8 gene and has been used to evaluate iPSC-derived cell therapies for haemophilia A. A functionally enhanced factor VIII gene integrated into the AAVS1 locus of patient-derived iPSCs was assessed in N2G-F8 mice.[6]
See also
[edit]References
[edit]Bibliography
[edit]- Kim, You-Min; Na, Hee Ju; Kwon, Do Hee; Lee, Jae Hoon; Park, Bo Min; Lee, Subin; Nam, Tae Wook; Park, Mi Yeon; Park, Sun Ha; Kim, Sung Joo; Choi, Bongkum; Lee, Han-Woong (2025). "Generation of NOD-SCID mice with near-complete deletions of Il2rg and Prkdc for human cancer and HSC engraftment". Transgenic Research. 34 (1): 35. doi:10.1007/s11248-025-00454-9. PMC 12254168. PMID 40643795.
- Choi, Yu-Ra; Hwang, Mihwa; Kim, Jaemin; Park, Seog-Yun; Kim, Sunshin; Song, Jae J; Lee, Youngjoo (2025). "Overcoming MET-targeted drug resistance in MET-amplified lung cancer by aurora kinase B inhibition". BBA Molecular Cell Research. 1872 (7) 120001. doi:10.1016/j.bbamcr.2025.120001. PMID 40499687.
- Song, Yaechan; Na, Heeju; Lee, Seung Eon; Kim, You Min; Moon, Jihyun; Nam, Tae Wook; Ji, Yul; Jin, Young; Park, Jae Hyung; Cho, Seok Chan; Lee, Jaehoon; Hwang, Daehee; Ha, Sang-Jun; Park, Hyun Woo; Kim, Jae Bum; Lee, Han-Woong (2024). "Dysfunctional adipocytes promote tumor progression through YAP/TAZ-dependent cancer-associated adipocyte transformation". Nature Communications. 15 (1) 4052. Bibcode:2024NatCo..15.4052S. doi:10.1038/s41467-024-48179-3. PMC 11094189. PMID 38744820.
- Kim, Do-Hun; Choi, Sang-Hwi; Sung, Jin Jea; Kim, Sieun; Yi, Hanui; Park, Sanghyun; Park, Chan Wook; Oh, Young Woo; Lee, Jungil; Kim, Dae-Sung; Kim, Jong-Hoon; Park, Chul-Yong; Kim, Dong-Wook (2025). "Long-term correction of hemophilia A via integration of a functionally enhanced FVIII gene into the AAVS1 locus by nickase in patient-derived iPSCs". Experimental and Molecular Medicine. 57 (1): 184–192. doi:10.1038/s12276-024-01375-z. PMC 11799516. PMID 39762408.
- "NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG)". The Jackson Laboratory. 2019.
- "CIEA NOG mouse®". Taconic Biosciences. 2024.
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