PIF1 5'-to-3' DNA helicase

PIF1
Identifiers
Aliases	PIF1, C15orf20, PIF, PIF1 5'-to-3' DNA helicase
External IDs	OMIM: 610953; MGI: 2143057; HomoloGene: 99775; GeneCards: PIF1; OMA:PIF1 - orthologs
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 5FHH
Enzyme activity EC # BRENDA ExPASy KEGG MetaCyc 5.6.2.3 ↗ ↗ ↗ ↗
Gene location (Human) Chr. Chromosome 15 (human) Band 15q22.31 Start 64,815,632 bp End 64,825,668 bp
Gene location (Mouse) Chr. Chromosome 9 (mouse) Band 9|9 C Start 65,587,160 bp End 65,595,967 bp
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in ventricular zone gonad ganglionic eminence stromal cell of endometrium mucosa of transverse colon appendix granulocyte bone marrow cell spleen lymph node Top expressed in otic vesicle hand Paneth cell ventricular zone primitive streak tail of embryo genital tubercle endothelial cell of lymphatic vessel hair follicle thymus More reference expression data BioGPS n/a
Gene ontology Molecular function nucleotide binding DNA binding telomeric DNA binding helicase activity DNA helicase activity single-stranded DNA helicase activity 5'-3' DNA/RNA helicase activity hydrolase activity ATP binding magnesium ion binding telomerase inhibitor activity 5'-3' DNA helicase activity G-quadruplex DNA binding Cellular component replication fork mitochondrion nucleus Biological process DNA recombination regulation of telomere maintenance negative regulation of telomerase activity G-quadruplex DNA unwinding cellular response to DNA damage stimulus telomere maintenance DNA repair DNA duplex unwinding mitochondrial genome maintenance DNA replication negative regulation of telomere maintenance via telomerase Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 80119 208084 Ensembl ENSG00000140451 ENSMUSG00000041064 UniProt Q9H611 Q80SX8 RefSeq (mRNA) NM_001286496 NM_001286497 NM_001286499 NM_025049 NM_172453 NM_001357526 NM_001357527 RefSeq (protein) NP_001273425 NP_001273426 NP_001273428 NP_079325 NP_766041 NP_001344455 NP_001344456 Location (UCSC) Chr 15: 64.82 – 64.83 Mb Chr 9: 65.59 – 65.6 Mb PubMed search
Wikidata
View/Edit Human View/Edit Mouse

PIF1 5'-to-3' DNA helicase is a protein that in humans is encoded by the PIF1 gene.

Function

This gene encodes a DNA-dependent adenosine triphosphate (ATP)-metabolizing enzyme that functions as a 5' to 3' DNA helicase. The encoded protein can resolve G-quadruplex structures and RNA-DNA hybrids at the ends of chromosomes. It also prevents telomere elongation by inhibiting the actions of telomerase. Alternative splicing and the use of alternative start codons results in multiple isoforms that are differentially localized to either the mitochondria or the nucleus. [provided by RefSeq, Nov 2013].

Further reading

• Zhang DH, Zhou B, Huang Y, Xu LX, Zhou JQ (2006). "The human Pif1 helicase, a potential Escherichia coli RecD homologue, inhibits telomerase activity". Nucleic Acids Res. 34 (5): 1393–404. doi:10.1093/nar/gkl029. PMC 1390689. PMID 16522649.
• Mateyak MK, Zakian VA (December 2006). "Human PIF helicase is cell cycle regulated and associates with telomerase". Cell Cycle. 5 (23): 2796–804. doi:10.4161/cc.5.23.3524. PMID 17172855.
• Futami K, Shimamoto A, Furuichi Y (September 2007). "Mitochondrial and nuclear localization of human Pif1 helicase". Biol. Pharm. Bull. 30 (9): 1685–92. doi:10.1248/bpb.30.1685. PMID 17827721.
• Gu Y, Masuda Y, Kamiya K (November 2008). "Biochemical analysis of human PIF1 helicase and functions of its N-terminal domain". Nucleic Acids Res. 36 (19): 6295–308. doi:10.1093/nar/gkn609. PMC 2577353. PMID 18835853.
• George T, Wen Q, Griffiths R, Ganesh A, Meuth M, Sanders CM (October 2009). "Human Pif1 helicase unwinds synthetic DNA structures resembling stalled DNA replication forks". Nucleic Acids Res. 37 (19): 6491–502. doi:10.1093/nar/gkp671. PMC 2770657. PMID 19700773.
• Sanders CM (August 2010). "Human Pif1 helicase is a G-quadruplex DNA-binding protein with G-quadruplex DNA-unwinding activity". Biochem. J. 430 (1): 119–28. doi:10.1042/BJ20100612. PMID 20524933.
• Gagou ME, Ganesh A, Thompson R, Phear G, Sanders C, Meuth M (July 2011). "Suppression of apoptosis by PIF1 helicase in human tumor cells". Cancer Res. 71 (14): 4998–5008. doi:10.1158/0008-5472.CAN-10-4404. PMID 21616935.
• Chisholm KM, Aubert SD, Freese KP, Zakian VA, King MC, Welcsh PL (2012). "A genomewide screen for suppressors of Alu-mediated rearrangements reveals a role for PIF1". PLOS ONE. 7 (2) e30748. Bibcode:2012PLoSO...730748C. doi:10.1371/journal.pone.0030748. PMC 3276492. PMID 22347400.
• Ramanagoudr-Bhojappa R, Chib S, Byrd AK, Aarattuthodiyil S, Pandey M, Patel SS, Raney KD (May 2013). "Yeast Pif1 helicase exhibits a one-base-pair stepping mechanism for unwinding duplex DNA". J. Biol. Chem. 288 (22): 16185–95. doi:10.1074/jbc.M113.470013. PMC 3668774. PMID 23596008.

External links

• Overview of all the structural information available in the PDB for UniProt: Q9H611 (ATP-dependent DNA helicase PIF1) at the PDBe-KB.

This article incorporates text from the United States National Library of Medicine, which is in the public domain.