Replication protein A3

RPA3
Identifiers
Aliases	RPA3, REPA3, RP-A p14, replication protein A3
External IDs	OMIM: 179837; MGI: 1915490; GeneCards: RPA3
Available structures
PDB	Ortholog search: PDBe RCSB
List of PDB id codes
	1L1O, 1QUQ, 2PI2, 2PQA, 2Z6K, 3KDF
Gene location (Human)
Chr.	Chromosome 7 (human)
End	7,718,607 bp
Gene location (Mouse)
Chr.	Chromosome 6 (mouse)
End	8,259,173 bp
RNA expression pattern
	Top expressed in
	bronchial epithelial cell; ; beta cell; ; trabecular bone; ; embryo; ; ventricular zone; ; bone marrow; ; olfactory zone of nasal mucosa; ; nasal epithelium; ; ganglionic eminence; ; mucosa of transverse colon;
	Top expressed in
	medial ganglionic eminence; ; otic placode; ; zygote; ; yolk sac; ; thymus; ; dermis; ; mandibular prominence; ; maxillary prominence; ; embryo; ; ventricular zone;
	More reference expression data
	More reference expression data
Gene ontology
Molecular function	single-stranded DNA binding; DNA binding; damaged DNA binding; protein binding;
Cellular component	nucleus; nucleoplasm; DNA replication factor A complex; site of double-strand break;
Biological process	DNA recombination; translesion synthesis; DNA replication; nucleotide-excision repair, DNA gap filling; transcription-coupled nucleotide-excision repair; regulation of cell population proliferation; nucleotide-excision repair, DNA incision; error-free translesion synthesis; cellular response to DNA damage stimulus; regulation of mitotic cell cycle; regulation of cellular response to heat; interstrand cross-link repair; error-prone translesion synthesis; nucleotide-excision repair, DNA incision, 5'-to lesion; nucleotide-excision repair, preincision complex stabilization; nucleotide-excision repair; DNA repair; base-excision repair; double-strand break repair via homologous recombination; nucleotide-excision repair, preincision complex assembly; telomere maintenance; telomere maintenance via semi-conservative replication; G1/S transition of mitotic cell cycle; DNA mismatch repair; nucleotide-excision repair, DNA incision, 3'-to lesion; regulation of signal transduction by p53 class mediator;
	Sources:Amigo / QuickGO
Orthologs
Databases	NCBI: entry; OMA: entry
	6119
	68240
	ENSG00000106399
	ENSMUSG00000012483
	P35244
	Q9CQ71
	NM_002947
	NM_026632
	NP_002938
	NP_080908
	Wikidata
View/Edit Human	View/Edit Mouse

Replication protein A 14 kDa subunit is a protein that in humans is encoded by the RPA3 gene.^[5]^[6] RPA is a single-stranded DNA-binding protein that is conserved in eukaryotes and plays essential roles in the metabolism of nucleic acids.^[7] Unlike helicase, RPA does not separate strands but binds and protects exposed single-stranded DNA to prevent secondary structure formation and degradation.^[8] RPA is usually considered to be the functional equivalent of bacterial single-stranded DNA-binding proteins (SSB), although it is much more complex structurally in eukaryotic cells.^[9]

Interactions

RPA3 has been shown to interact with replication protein A1^[10]^[11] and replication protein A2.^[10]^[11] Together, they form a heterotrimeric complex that contributes to the direct binding of single-stranded DNA during replication, homologous recombination, nucleoetide excision repair, and mismatch repair.^[12] RPA3 can directly contact ssDNA on the 3' side of a substrate, and this polarity is crucial for the positioning and stability of nucleases that are involved in excision repair^[13]

References

1 2 3 GRCh38: Ensembl release 89: ENSG00000106399 – Ensembl, May 2017
1 2 3 GRCm38: Ensembl release 89: ENSMUSG00000012483 – Ensembl, May 2017
↑ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
↑ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
↑ Umbricht CB, Erdile LF, Jabs EW, Kelly TJ (March 1993). "Cloning, overexpression, and genomic mapping of the 14-kDa subunit of human replication protein A". The Journal of Biological Chemistry. 268 (9): 6131–6138. doi:10.1016/S0021-9258(18)53229-4. PMID 8454588.
↑ "Entrez Gene: RPA3 replication protein A3, 14kDa".
↑ Salas TR, Petruseva I, Lavrik O, Saintomé C (January 2009). "Evidence for direct contact between the RPA3 subunit of the human replication protein A and single-stranded DNA". Nucleic Acids Research. 37 (1): 38–46. doi:10.1093/nar/gkn895. PMC 2615627. PMID 19010961.
↑ Chen R, Wold MS (December 2014). "Replication protein A: single-stranded DNA's first responder: dynamic DNA-interactions allow replication protein A to direct single-strand DNA intermediates into different pathways for synthesis or repair". BioEssays: News and Reviews in Molecular, Cellular and Developmental Biology. 36 (12): 1156–1161. doi:10.1002/bies.201400107. PMC 4629251. PMID 25171654.
↑ Iftode C, Daniely Y, Borowiec JA (1999). "Replication protein A (RPA): the eukaryotic SSB". Critical Reviews in Biochemistry and Molecular Biology. 34 (3): 141–180. doi:10.1080/10409239991209255. PMID 10473346.
1 2 Bochkareva E, Frappier L, Edwards AM, Bochkarev A (February 1998). "The RPA32 subunit of human replication protein A contains a single-stranded DNA-binding domain". The Journal of Biological Chemistry. 273 (7): 3932–3936. doi:10.1074/jbc.273.7.3932. PMID 9461578.
1 2 Bochkareva E, Korolev S, Lees-Miller SP, Bochkarev A (April 2002). "Structure of the RPA trimerization core and its role in the multistep DNA-binding mechanism of RPA". The EMBO Journal. 21 (7): 1855–1863. doi:10.1093/emboj/21.7.1855. PMC 125950. PMID 11927569.
↑ Madru C, Martínez-Carranza M, Laurent S, Alberti AC, Chevreuil M, Raynal B, et al. (April 2023). "DNA-binding mechanism and evolution of replication protein A". Nature Communications. 14 (1) 2326. Bibcode:2023NatCo..14.2326M. doi:10.1038/s41467-023-38048-w. PMC 10122647. PMID 37087464.
↑ de Laat WL, Appeldoorn E, Sugasawa K, Weterings E, Jaspers NG, Hoeijmakers JH (August 1998). "DNA-binding polarity of human replication protein A positions nucleases in nucleotide excision repair". Genes & Development. 12 (16): 2598–2609. doi:10.1101/gad.12.16.2598. PMC 317078. PMID 9716411.

Keshav KF, Chen C, Dutta A (June 1995). "Rpa4, a homolog of the 34-kilodalton subunit of the replication protein A complex". Molecular and Cellular Biology. 15 (6): 3119–3128. doi:10.1128/mcb.15.6.3119. PMC 230543. PMID 7760808.
Umbricht CB, Griffin CA, Hawkins AL, Grzeschik KH, O'Connell P, Leach R, et al. (March 1994). "High-resolution genomic mapping of the three human replication protein A genes (RPA1, RPA2, and RPA3)". Genomics. 20 (2): 249–257. doi:10.1006/geno.1994.1161. PMID 8020972.
Amacker M, Hottiger M, Mossi R, Hübscher U (March 1997). "HIV-1 nucleocapsid protein and replication protein A influence the strand displacement DNA synthesis of lentiviral reverse transcriptase". Aids. 11 (4): 534–536. PMID 9084803.
Sanger Centre T, Washington University Genome Sequencing Cente T (November 1998). "Toward a complete human genome sequence". Genome Research. 8 (11): 1097–1108. doi:10.1101/gr.8.11.1097. PMID 9847074.
Mer G, Bochkarev A, Gupta R, Bochkareva E, Frappier L, Ingles CJ, et al. (October 2000). "Structural basis for the recognition of DNA repair proteins UNG2, XPA, and RAD52 by replication factor RPA". Cell. 103 (3): 449–456. doi:10.1016/S0092-8674(00)00136-7. PMID 11081631. S2CID 16640087.
Habel JE, Ohren JF, Borgstahl GE (February 2001). "Dynamic light-scattering analysis of full-length human RPA14/32 dimer: purification, crystallization and self-association". Acta Crystallographica. Section d, Biological Crystallography. 57 (Pt 2): 254–259. Bibcode:2001AcCrD..57..254H. doi:10.1107/S0907444900015225. PMID 11173472.
Kneissl M, Pütter V, Szalay AA, Grummt F (March 2003). "Interaction and assembly of murine pre-replicative complex proteins in yeast and mouse cells". Journal of Molecular Biology. 327 (1): 111–128. doi:10.1016/S0022-2836(03)00079-2. PMID 12614612.
Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, et al. (October 2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173–1178. Bibcode:2005Natur.437.1173R. doi:10.1038/nature04209. PMID 16189514. S2CID 4427026.
Ewing RM, Chu P, Elisma F, Li H, Taylor P, Climie S, et al. (2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry". Molecular Systems Biology. 3 (1) 89. doi:10.1038/msb4100134. PMC 1847948. PMID 17353931.
Sankaranarayanan P, Schomay TE, Aiello KA, Alter O (April 2015). "Tensor GSVD of patient- and platform-matched tumor and normal DNA copy-number profiles uncovers chromosome arm-wide patterns of tumor-exclusive platform-consistent alterations encoding for cell transformation and predicting ovarian cancer survival". PLOS ONE. 10 (4) e0121396. Bibcode:2015PLoSO..1021396S. doi:10.1371/journal.pone.0121396. PMC 4398562. PMID 25875127. AAAS EurekAlert! Press Release and NAE Podcast Feature.

This article on a gene on human chromosome 7 is a stub. You can help Wikipedia by adding missing information.

[refGRCh38Ensembl-1] 1 2 3 GRCh38: Ensembl release 89: ENSG00000106399 – Ensembl, May 2017

[refGRCm38Ensembl-2] 1 2 3 GRCm38: Ensembl release 89: ENSMUSG00000012483 – Ensembl, May 2017

[3] "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[4] "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[Umbricht_1993-5] Umbricht CB, Erdile LF, Jabs EW, Kelly TJ (March 1993). "Cloning, overexpression, and genomic mapping of the 14-kDa subunit of human replication protein A". The Journal of Biological Chemistry. 268 (9): 6131–6138. doi:10.1016/S0021-9258(18)53229-4. PMID 8454588.

[entrez-6] "Entrez Gene: RPA3 replication protein A3, 14kDa".

[7] Salas TR, Petruseva I, Lavrik O, Saintomé C (January 2009). "Evidence for direct contact between the RPA3 subunit of the human replication protein A and single-stranded DNA". Nucleic Acids Research. 37 (1): 38–46. doi:10.1093/nar/gkn895. PMC 2615627. PMID 19010961.

[8] Chen R, Wold MS (December 2014). "Replication protein A: single-stranded DNA's first responder: dynamic DNA-interactions allow replication protein A to direct single-strand DNA intermediates into different pathways for synthesis or repair". BioEssays: News and Reviews in Molecular, Cellular and Developmental Biology. 36 (12): 1156–1161. doi:10.1002/bies.201400107. PMC 4629251. PMID 25171654.

[9] Iftode C, Daniely Y, Borowiec JA (1999). "Replication protein A (RPA): the eukaryotic SSB". Critical Reviews in Biochemistry and Molecular Biology. 34 (3): 141–180. doi:10.1080/10409239991209255. PMID 10473346.

[Bochkareva_1998-10] 1 2 Bochkareva E, Frappier L, Edwards AM, Bochkarev A (February 1998). "The RPA32 subunit of human replication protein A contains a single-stranded DNA-binding domain". The Journal of Biological Chemistry. 273 (7): 3932–3936. doi:10.1074/jbc.273.7.3932. PMID 9461578.

[Bochkareva_2002-11] 1 2 Bochkareva E, Korolev S, Lees-Miller SP, Bochkarev A (April 2002). "Structure of the RPA trimerization core and its role in the multistep DNA-binding mechanism of RPA". The EMBO Journal. 21 (7): 1855–1863. doi:10.1093/emboj/21.7.1855. PMC 125950. PMID 11927569.

[12] Madru C, Martínez-Carranza M, Laurent S, Alberti AC, Chevreuil M, Raynal B, et al. (April 2023). "DNA-binding mechanism and evolution of replication protein A". Nature Communications. 14 (1) 2326. Bibcode:2023NatCo..14.2326M. doi:10.1038/s41467-023-38048-w. PMC 10122647. PMID 37087464.

[13] Laat WL, Appeldoorn E, Sugasawa K, Weterings E, Jaspers NG, Hoeijmakers JH (August 1998). "DNA-binding polarity of human replication protein A positions nucleases in nucleotide excision repair". Genes & Development. 12 (16): 2598–2609. doi:10.1101/gad.12.16.2598. PMC 317078. PMID 9716411.

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Replication protein A3

Interactions

See also

References

Further reading